However, we predominantly found enrichment in Fabsialylated IgG and not for Fc-sialylated IgG. Further analysis revealed that SNA lectin binding to the Fc part only occurred under non-native conditions. From the literature it is known that monosialylation of the Fc part is not sufficient for Fc binding to SNA lectin. Thus,WY 14643 our results indicate that IVIg contains predominantly IgG with monosialylated Fc parts which are not available for SNA lectin binding under native conditions and thereby did not result in a substantial increase of Fc-sialylation in IVIg. Nevertheless, it was suggested by Stadlmann et al. that enrichment by SNA lectin chromatography is limited to disialylated Fc parts,WZ4002 a fraction too small to detect but arguably responsible for the immune modulating effects of IVIg as found by Kaneko et al. Because we essentially enriched for sialic acid similar to Kaneko et al., we propose that the lack of efficacy of IVIg-SA in our model points to a different mechanism of action of IVIg in the PIT model compared to the murine K/N serum transfer model for rheumatoid arthritis. Anglers in the Pacific Northwest and the Midwest states of the U.S. often use eggs that are cured with a mix of preservatives, dyes, salts, and sugars as bait for trout and salmon. These eggs may either be purchased pre-cured or anglers can purchase the cures or cure ingredients and prepare their own eggs. Although anglers are typically targeting adult fish, juvenile salmonids are known to consume cured eggs incidentally. In the Pacific Northwest states, anglers typically cure the full ovary and cut it into smaller pieces which are attached to a hook. Conversely, anglers in theMidwest of the US often use net bags to contain the eggs, though there is likely still some level of exposure to smolts from chumming and/or splitting of the bags. The level of exposure experienced by individual juvenile fish is unknown, but in areas of heavy fishing pressure it is potentially quite high. Factors such as the number of anglers fishing cured eggs, the time spent fishing in the home range or vicinity of an individual fish, the appetite of the fish, and the density of fish in an area could all affect the level of exposure. Despite the potential for exposure, there has been little consideration for the effects of cured eggs on the health of juvenile salmonids.