Thus, Scd1 is an attractive target for the treatment of many metabolic disorders. In the present study, we observed a sharp upregulation of Scd1 mRNA and protein immediately after weaning, corroborating earlier reports. However, which factors regulate Scd1 during weaning is unknown. Scd1 expression in adult cells is regulated by many transcription factors including Srebp1c, Ppara, C/EBP-a, Pgc1-a, and LXRa. From our data, LXRa is the only one of the above mentioned transcription factors whose mRNA expression was highest after weaning. These data suggest that LXRa may potentially regulate Scd1 expression at weaning; however, further research is needed to confirm this. The mRNA expressions of many enzymes of cholesterol biosynthesis followed two major patterns of expression: either decreased expression during the Oxybenzone suckling period, which is similar to hepatic cholesterol metabolism observed in sheep ; or induced expression at weaning. The fact that many genes followed the same pattern of expression suggests a common mechanism of regulation. Srebp2 along with its regulatory Insig proteins is known to regulate the transcription of cholesterol metabolism genes in adults. Srebp2 mRNA expression did not follow either of the above mentioned gene expression patterns, suggesting other factors may be controlling cholesterol metabolism gene expression during development. Determining these factors may provide novel therapeutic targets for the control of cholesterol disorders. RNA-sequencing provides an unbiased detection of transcripts. Thus, we were able to quantitatively compare the transcript Radotinib abundances of known isoforms of genes throughout liver development. Interestingly, the alternative splicing isoforms of both pyruvate carboxylase and pyruvate kinase, liver showed age dependent switches in isoform expression dominance. We are the first to reveal that isoform 2 of Pklr is the dominant form in the adult ages; however, isoform 1 is more dominantly expressed during the neonatal ages.Additionally, isoform 2 of Pcx is only dominantly expressed over isoform 1 during the early suckling ages.