The timing of the commencement of olfactory imprinting in juvenile salmon before PST should be examined from the alevin stage. In conclusion, the Crassicauline-A present study shows that one-year-old lacustrine sockeye salmon can be imprinted by a single amino acid. The olfactory imprinting occurs before and during PST, but not after PST. The odorant memories of one amino acid are maintained not only in the spawning season but also in the nonspawning season. The requirement time for imprinting is likely to based multicalc software. In order to examine whether the fish were imprinted by Pro or Glu, EOG responses to the test water were compared between the experimental fish and the control fish. The EOG response was measured according to the technique of Evans and Hara in June and October from 2005 to 2007 in the monthly variation experiment, and in June and October 2006 and 2007 in the requirement time experiment. Fish were immobilized with an intramuscular injection of 3 mg/kg body weight gallamine triethodide. Gills were aerated through the mouth with an aerated solution of clove oil, which was not allowed to contact olfactory rosettes. The responsive properties of olfactory receptor cells were recorded by using a pair of glass microelectrodes filled with 2.5% agar-saline and bridged to silver wire. With the aid of a stereomicroscope and micromanipulators, an odorant perfusion tube was inserted gently into the in-current passage of the noses, and the recording microelectrode was inserted through the excurrent passage and positioned above the midline of the rosette at the base of posterior-most lamella. ET-743 has a broad spectrum of activity in tumor cell lines at pM and low nM concentrations, and it also has clinical activity towards ovarian cancer, breast cancer, and sarcomas. ET743 has been approved by the EMEA/EU for patients with advanced sarcomas who have either progressed after treatment with an anthracycline or are not clinically suitable to receive conventional agents. ET-743 is composed of three tetrahydroisoquinoline subunits containing a central carbinolamine moiety enabling it to Benzoylhypacoitine covalently bind to DNA.