The detection limit of Tenacissoside-G SINBAD is 10 pM, which is in the range of other sensitive methods, but does not reach the sensitivity of antibody-or conductivity-based assays. However, the power of SINBAD does not rely on the detection of single nano-entities. SINBAD is compatible with standard pull-down reagents and does not require the development of miniaturized devices. SINBAD combines the affinity tag platforms, which are widely used for protein purification, with the single molecule detection sensitivity of fluorescence microscopy. Thereby SINBAD overcomes one of the major limitations of pull-down assays, i.e. the requirement of micrograms of proteins. A similar approach to detect protein-protein interactions by fluorescence microscopy has recently been used to study low affinity interactions between proteins by fluorescent microscopy. This ��bead halo�� method detects binding of fluorescently labeled proteins in real time under equilibrium binding conditions without washing steps. While the ��bead halo�� method works well for low affinity interactions, SINBAD successfully combines the ability to study high affinity interactions and to reduce the amount of protein needed for a single experiment. This is relevant since many proteins, which cannot be expressed in E. coli or other cellular expression systems, can often be translated at low levels in cell-free systems. Additionally, SINBAD offers a tool to study protein interactions from as little as 50 cells. Second, color-coding of proteins with QDs of different emission wave lengths offers the unique opportunity to monitor multiple binding events in a single experiment on a single bead. We expect that SINBAD can be expanded to other classes of molecules as long as they can be conjugated to QDs. These molecules include but are not limited to RNA, peptides, metabolites or small chemical compounds. Finally, SINBAD provides both a screening platform for identifying new biomolecular interactions as well as an experimental system to characterize the molecular, dynamic and energetic nature of these interactions. Since most laboratories are capable of performing fluorescent microscopy, SINBAD can be easily implemented. Although not tested here, SINBAD Cefmenoxime HCl should be adaptable for highthroughput setups using large-scale magnetic separation systems. Neuroblastoma, like most human cancers, is characterized by genomic instability, manifested at the chromosomal level as allelic gain, loss, or rearrangement.