We speculate that this tendency is observed because prolonged Moexipril HCl duration of ischemic time stimulates upregulation of various proand antioxidative enzymes, rather than generating free radicals. In our recent paper, we have demonstrated that during kidney I/R injury, upregulation of xanthine oxidoreductase activity occurs and is most pronounced in patients with the longest CIT values. Nevertheless, although we have established a direct association between XOR activity and intensity of isoprostane levels, in the present study, we did not Ascomycin observe a correlation between CIT and isoprostane concentrations. Therefore, our results suggest that while the influence of CIT on modulation of postoperative allograft function is inherent, its effect on perioperative changes in oxidative stress intensity seems to be indirect and probably modulated by the recipient��s antioxidative potential. Finally, the clinical value of platelet antioxidant activity/levels as diagnostic markers for SGF/DGF prediction must be emphasized. In our study, we have demonstrated that several platelet antioxidants may help differentiate between EGF and SGF/DGF. Among these parameters, catalase and G6P activity seems to be of the greatest diagnostic value for SGF/DGF prediction. Thus far, several authors have discovered clinically suitable early post-transplant markers for SGF/DGF prediction ; however, our team concentrates on finding pre- and/or perioperative SGF/DGF predictors. In the current study, we described novel pre- and perioperative markers that have a sensitivity of up to 80�C90% and therefore seem to be promising tools for prediction of post-transplant outcome in the future. Unfortunately, at this stage of our research, these markers do not seem suitable for independent decision making because of their low specificity. Moreover, their cut-off and predictive values must be verified in further cohort studies. Nevertheless, our study demonstrated that the early phase of kidney allograft reperfusion is associated with intensified oxidative stress and is accompanied by significant changes in the activity/ levels of platelet antioxidants, whose actions may contribute to limitation of the perioperative intensity of oxidative stress. Our study also highlighted the lack of ����full��’activity of platelet antioxidative armament as a potential biochemical explanation for problems associated with post-transplant graft activation. Finally, we also demonstrated that the perioperative activity of selected platelet antioxidants may help differentiate between EGF and SGF/DGF. Guidelines for the prevention of coronary heart disease recommend the use of risk scores to identify adults at higher risk of CHD for whom preventive therapy�Ce.g., by lipid lowering drugs�C has higher absolute benefits. Several scoring systems exist to help clinicians assess the 10-year CHD risk, with the Framingham risk score the most widely used.