Our transcriptomics data are particularly relevant in the context of recent research detailing the effect of quorum sensing on the polymicrobial interactions of other respiratory bacterial pathogens. In particular, a recent study by Armbruster et al. showed that co-colonization of M. catarrhalis and H. influenzae results in increased M. catarrhalis biofilm formation and resistance to antibiotics.Enhanced interfacial properties were observed for construct-tonative but not for the native-to-native case. Group D, which was treated with LOX for the longest period of time, had statistically higher tensile properties at the interface than did the other three groups. Specifically, Group D had approximately 2.2 times the tensile strength of controls. This was confirmed with morphological and histological data. The results of this study are significant for both current and prospective cartilage regeneration and repair methods. It is worth noting that, despite the lack of any significant differences in the collagen and GAG content in either the construct or explant groups, there were significant LOX-induced increases in interface biomechanics. The fact that interfacial mechanical properties increased significantly in the absence of increases in the main extracellular matrix components suggests that cross-links play a central role in integration. Unfortunately, a relationship between the strength of the interface and the number of cross-links at the interface variability mdd crhr1 gene antidepressant response mdd cannot be directly assessed. This is because the interface cannot be isolated without adjacent tissues that, too, contain cross-links. It is, therefore, difficult to ascertain the fraction of cross-links belonging to the interface alone. The same can be said of the collagen and GAG production by chondrocytes at the interface. Since the interface consists of a thin layer, minute changes in the ECM of this area would be masked by the comparatively greater ECM content of the cartilages undergoing integration. The mechanism of LOX-induced collagen crosslinking is well-established and a strong candidate for explaining the results obtained in this study, though this was not directly proven here. Bolstering this hypothesis, recent studies have shown induced collagen crosslinks in engineered cartilage improves tensile stiffness. Future studies may consider techniques such as time-resolved fluorescence spectroscopy to quantify PYR at the interface. Neuropeptide Y in particular stands out as a prime candidate for sensing and responding to signals of energy homeostasis as NPY expression levels respond to both short-term and long-term fasting conditions.