Genes that were highly downregulated in abundance between early and late blood-fed midges were lipases, takeout-like protein, pyruvate metabolism, vitellogenins, and attacins suggesting these processes were either required or induced earlier in the blood metabolism process. Genes that were highly upregulated between the early and late blood-fed conditions including chitinases, trypsins, serine collagenases, tankyrase, other lipases, salivary proteins, and many other digestion related genes suggests they were utilized later in the blood digestion process. In comparison to the blood feeding process, and in congruence with the stark contrasts seen in Figs. 5–7, the genetic response to sucrose feeding was unremarkable, where both the early sucrose and late sucrose transcriptomes were relatively similar to the nonfeeding teneral state. We observed only 140 differentially expressed genes right after a sucrose meal, and 1,149 at 36 hours post feeding, compared to teneral midges. Categories containing genes with altered expression profiles that responded to early sucrose feeding were anatomical structure development, embryo development, DNA binding, and response to stress. In the 36 h post-sucrose transcriptome, we observed a larger physiological response and a general trend in transcript abundance decrease in anatomical structure development, cell differentiation, ion binding, reproduction, and signal transduction categories when compared to teneral midges, with the exception of a net abundance increase in the oxidoreductase superfamily, which has been previously described with salivary activity in insects. Comparison of sucrose feeding over time, showed a decrease in the expression of ion binding genes, and a significant increase in genes classified as anatomical structure development, cellular nitrogen metabolic process, small molecule metabolic process, oxidoreductase activity, and transferase activity. Unlike many other important hematophagous insect vectors, the genome and transcriptome of C. sonorensis have not been available as a resource for Culicoides vector biologists, which has hindered genetic and functional genomics studies as well as detailed understanding of molecular, cellular and physiological processes of this important vector. The adult female reference transcriptome and differential expression analysis presented here represents a critical milestone and fills a profound gap in C. sonorensis biology such as understanding the genetic basis of anautogeny, hematophagy and other key physiological processes specific to the midge, and is paramount for the development of novel approaches to vector control. Tigecycline is a first-in-class glycylcycline antibiotic, developed as a third generation structural analog of older tetracyclines. It displays broad-spectrum, potent activity against both Grampositive and Gram-negative bacteria, including many multidrugresistant.